Calponin 1 Antibody (CNN1/4227R) [DyLight 488]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08462G

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # CNN1/4227R

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Calponin 1 Antibodies »

Product Summary for Calponin 1 Antibody (CNN1/4227R) [DyLight 488]

Immunogen

Synthetic peptide corresponding to residues on the C-terminus of human Calponin 1 was used as an immunogen. (Uniprot: P51911)

Localization

Cytoplasmic

Specificity

Calponin a 33 kDa polypeptide, is a cytoskeleton-associated actin-binding protein that also interacts tropomyosin and calmodulin. Calponin antibody has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast. Calponin antibody may also have applications in malignant myoepithelium and pleomorphic adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions. The antibody has been found in tissue extracts from smooth muscle, but not in fibroblast extracts.

Marker

Smooth Muscle Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Calponin 1 Antibody (CNN1/4227R) [DyLight 488]

Application
Recommended Usage

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Calponin 1

Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction (1). Calponin binds filamentous actin (F-actin) through two distinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2) Immunoreactivity for calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas (3).

Alternate Names

Calponin H1, CNN1, Sm-Calp, SMCC

Gene Symbol

CNN1

Additional Calponin 1 Products

Product Documents for Calponin 1 Antibody (CNN1/4227R) [DyLight 488]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Calponin 1 Antibody (CNN1/4227R) [DyLight 488]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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