cAMP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2146
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry
Cited:
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 250532
Product Specifications
Immunogen
Thryoglobulin-coupled cAMP
Specificity
Detects cAMP. In a competition binding assay, this antibody does not cross-react with ATP, CTP, GTP, AMP, GMP, cGMP, or cUMP.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for cAMP Antibody
cAMP in Human PBMCs.
cAMP was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-cAMP Monoclonal Antibody (Catalog # MAB2146) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for cAMP Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: cAMP
Long Name
Cyclic AMP
Alternate Names
Cyclic AMP
Additional cAMP Products
Product Documents for cAMP Antibody
Product Specific Notices for cAMP Antibody
For research use only
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