Canine CD4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2410
Key Product Details
Species Reactivity
Canine
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant canine CD4
Val25-Lys401
Accession # P33705
Val25-Lys401
Accession # P33705
Specificity
Detects canine CD4 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant feline CD4 is observed and 5% cross-reactivity with recombinant human CD4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Canine CD4 Antibody
CD4 in Canine PBMCs.
CD4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2410) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Canine CD4 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Canine peripheral blood mononuclear cells
Sample: Canine peripheral blood mononuclear cells
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed canine peripheral blood mononuclear cells (PBMCs)
Sample: Immersion fixed canine peripheral blood mononuclear cells (PBMCs)
Western Blot
0.1 µg/mL
Sample: Recombinant Canine CD4
Sample: Recombinant Canine CD4
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD4
Alternate Names
CD4
Entrez Gene IDs
Gene Symbol
CD4
UniProt
Additional CD4 Products
Product Documents for Canine CD4 Antibody
Product Specific Notices for Canine CD4 Antibody
For research use only
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