Canine CD4 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2410
Key Product Details
Species Reactivity
Canine
Applications
Flow Cytometry, Immunocytochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant canine CD4
Val25-Lys401
Accession # P33705
Val25-Lys401
Accession # P33705
Specificity
Detects canine CD4 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant feline CD4 is observed, 5% cross‑reactivity with recombinant human CD4 is observed, and less than 1% cross-reactivity with recombinant mouse CD4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Canine CD4 Biotinylated Antibody
CD4 in Canine PBMCs.
CD4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine CD4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2410) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Canine CD4 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Canine peripheral blood mononuclear cells
Sample: Canine peripheral blood mononuclear cells
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed canine peripheral blood mononuclear cells
Sample: Immersion fixed canine peripheral blood mononuclear cells
Western Blot
0.1 µg/mL
Sample: Recombinant Canine CD4
Sample: Recombinant Canine CD4
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD4
Alternate Names
CD4
Entrez Gene IDs
Gene Symbol
CD4
UniProt
Additional CD4 Products
Product Documents for Canine CD4 Biotinylated Antibody
Product Specific Notices for Canine CD4 Biotinylated Antibody
For research use only
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