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Canine IL-18/IL-1F4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2924

R&D Systems, part of Bio-Techne
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MAB2924
MAB2924-SP

Key Product Details

Species Reactivity

Canine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 314820

Product Specifications

Immunogen

E. coli-derived recombinant canine IL‑18/IL‑1F4
Tyr37-Ser193
Accession # Q9XSR0

Specificity

Detects canine IL‑18/IL‑1F4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-18, recombinant mouse (rm) IL-18, recombinant porcine IL-18, recombinant rat IL-18, recombinant rhesus macaque IL-18, rhIL‑36Ra, rhIL-1F7, rhIL-36 gamma, rhIL-1F10, rmIL-1ra, rmIL-36 gamma, or rmIL-36 alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Canine IL-18/IL-1F4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Canine IL-18/IL-1F4

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18/IL-1F4

Interleukin 18, also known as interferon-gamma-inducing factor (IGIF) or IL-1 gamma, is an 18 kDa member of the IL-1 superfamily which shares biologic activities with IL‑12. IL-18 is expressed by liver cells, monocyte/macrophages, osteoblasts and keratinocytes. Mature canine IL-18 shares 73% sequence identity with human IL-18. Caspase-1 (IL-1 beta-converting enzyme) has been shown to process pro-IL-18 to active IL-18. Human IL-18 has been shown to enhance NK cell activity, induce the production of IFN-gamma and GM-CSF and inhibit the production of IL-10 by PBMCs.

Long Name

Interleukin 18

Alternate Names

IGIF, IL-1F4, IL-1g, IL18

Entrez Gene IDs

3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)

Gene Symbol

IL18

UniProt

Additional IL-18/IL-1F4 Products

Product Documents for Canine IL-18/IL-1F4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Canine IL-18/IL-1F4 Antibody

For research use only

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