Canine IL-1 beta/IL-1F2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3747
Key Product Details
Species Reactivity
Validated:
Canine
Cited:
Canine
Applications
Validated:
CyTOF-ready, Intracellular Staining by Flow Cytometry, Neutralization, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant canine IL-1 beta/IL-1F2
Ala114-Ser265
Accession # ABB73214
Ala114-Ser265
Accession # ABB73214
Specificity
Detects canine IL-1 beta in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Canine IL-1 beta/IL-1F2 Antibody
Cell Proliferation Induced by IL‑1 beta/IL‑1F2 and Neutral-ization by Canine IL-1 beta/IL-1F2 Antibody.
Recombinant Canine IL-1 beta/IL-1F2 (Catalog # 3747-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-1 beta/IL-1F2 (20 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-1 beta/IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3747). The ND50 is typically 2-10 µg/mL.Applications for Canine IL-1 beta/IL-1F2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Canine peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Sample: Canine peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Western Blot
0.1 µg/mL
Sample: Recombinant Canine IL-1 beta/IL-1F2 (Catalog # 3747-CL)
Sample: Recombinant Canine IL-1 beta/IL-1F2 (Catalog # 3747-CL)
Neutralization
Measured by its ability to neutralize IL‑1 beta/IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 20 pg/mL Recombinant Canine IL‑1 beta/IL‑1F2.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 22% amino acid (aa) identity in dog. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The canine IL-1 beta cDNA encodes a 266 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta-converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature canine IL-1 beta shares 68-78% aa sequence identity with cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus macaque IL-1 beta.
References
- Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
- Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
- Accession # NP_001033060.
- Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Long Name
Interleukin 1 beta
Alternate Names
IL-1b, IL-1F2, IL1 beta, IL1B
Entrez Gene IDs
Gene Symbol
IL1B
UniProt
Additional IL-1 beta/IL-1F2 Products
Product Documents for Canine IL-1 beta/IL-1F2 Antibody
Product Specific Notices for Canine IL-1 beta/IL-1F2 Antibody
For research use only
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