Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Carm1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide that maps to a region between residues 450 and 500 of human Coactivator-Associated Arginine Methyltransferase 1 using the numbering given in Swiss-Prot entry Q86X55 (GeneID 10498).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Carm1 Antibody

Western Blot: Carm1 Antibody [NB200-341]

Western Blot: Carm1 Antibody [NB200-341]

Western Blot: Carm1 Antibody [NB200-341] - Detection of Human and Mouse CARM1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody NB200-341 used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-341]

Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-341]

Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-341] - Human stomach carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
Western Blot: Carm1 Antibody [NB200-341]

Western Blot: Carm1 Antibody [NB200-341]

Western Blot: Carm1 Antibody [NB200-341] - Whole HeLa cell lysate (25 mcg for WB in A; 5 mg for IP in B).antibody used at 0.1 mcg/ml for WB and at 4 mcg/5 mg lysate for IP. Detection: Chemiluminescence A. Coomassie stain in B. Band was verified by mass spectrometry.

Applications for Carm1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

1-4 ug/mg lysate

Western Blot

1:5000-1:15000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Carm1

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT4 belongs to a family of proteins that catalyzes the methylation of arginine residues. The PRMT proteins are recruited by C-terminal activation domain 2 of the SRC (steroid receptor coactivator) GRIP1, whereas p300 interacts with activation domain 1 in the C terminus. CARM1 can methylate histone H3 in vitro, and a mutation in the putative S-adenosylmethionine binding domain of CARM1 substantially reduces both methyltransferase and coactivator activities suggesting that coactivator mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation.

Long Name

Coactivator-associated Arginine Methyltransferase 1

Alternate Names

PRMT4

Entrez Gene IDs

10498 (Human)

Gene Symbol

CARM1

UniProt

Q86X55 (Human)

Additional Carm1 Products

Product Documents for Carm1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Carm1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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