Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Carm1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide that maps to a region between residues 550 and the C-terminus (residue 585) of human Coactivator-Associated Arginine Methyltransferase 1 using the numbering given in Swiss-Prot entry Q86X55 (GeneID 10498).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Carm1 Antibody

Knockdown Validated: Carm1 Antibody [NB200-342]

Knockdown Validated: Carm1 Antibody [NB200-342]

Knockdown Validated: Carm1 Antibody [NB200-342] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Carm1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:5000 ug/ml of Rabbit Anti-Human Carm1 Polyclonal Antibody (Catalog # NB200-342) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Carm1 at approximately 65 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
Western Blot: Carm1 Antibody [NB200-342]

Western Blot: Carm1 Antibody [NB200-342]

Western Blot: Carm1 Antibody [NB200-342] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-342]

Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-342]

Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-342] - Detection of human CARM1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CARM1 (Cat. No. A300-421A lot 4) used at 1:1,000 (1ug/ml). Detection: DAB
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Applications for Carm1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg lysate

Knockdown Validated

1:5000

Western Blot

1:10000 - 1:25000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. In some cases, the antibody may be diluted further than indicated. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Carm1

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT4 belongs to a family of proteins that catalyzes the methylation of arginine residues. The PRMT proteins are recruited by C-terminal activation domain 2 of the SRC (steroid receptor coactivator) GRIP1, whereas p300 interacts with activation domain 1 in the C terminus. CARM1 can methylate histone H3 in vitro, and a mutation in the putative S-adenosylmethionine binding domain of CARM1 substantially reduces both methyltransferase and coactivator activities suggesting that coactivator mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation.

Long Name

Coactivator-associated Arginine Methyltransferase 1

Alternate Names

PRMT4

Entrez Gene IDs

10498 (Human)

Gene Symbol

CARM1

UniProt

Q86X55 (Human)

Additional Carm1 Products

Product Documents for Carm1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Carm1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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