Caspase-11 Antibody (17D9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80612

Novus Biologicals, part of Bio-Techne
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NBP2-80612
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Catalog #
BSA Free
NB120-10454
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Knockout Validated, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 17D9

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Caspase-11 Antibodies »

Product Summary for Caspase-11 Antibody (17D9) - Azide and BSA Free

Immunogen

A recombinant p30 subunit of mouse Caspase 11. [UniProt# P70343]

Localization

Cytoplasmic (Endoplasmic reticulum membrane and Mitochondrion)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-11 Antibody (17D9) - Azide and BSA Free

Western Blot: Caspase-11 Antibody (17D9)Azide and BSA Free [NBP2-80612]

Western Blot: Caspase-11 Antibody (17D9)Azide and BSA Free [NBP2-80612]

Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Mouse liver extract was separated on a 4-15% gel and transfered to PVDF membrane. The membrane was probed with anti-caspase 11 antibody and detected with an anti-mouse HRP secondary antibody. Both procaspase 11 and fully cleaved caspase 11 are detected. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]

Immunocytochemistry/ Immunofluorescence: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]

Immunocytochemistry/Immunofluorescence: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Caspase 11 antibody was tested in NIH-3T3 cells at a 1:100 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI. Image from the standard format of this antibody.
Immunohistochemistry: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]

Immunohistochemistry: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]

Immunohistochemistry: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse lung using Caspase 11 antibody (clone 17D9) at 1:100 diluton. The antibody generated cytoplasmic signal with more intense staining in the bronchiolar epithelium while the stainin
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Applications for Caspase-11 Antibody (17D9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 27708283)

Immunoblotting

reported in scientific literature (PMID 28345580)

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

reported in scientific literature (PMID 10791975)

Western Blot

5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-11

Caspases are cysteine proteases that play an essential role in apoptosis, and their regulation is post-translational to enable rapid activation. Murine Caspase 11, also known as Caspase 4 or ICH3, has a heterotetrameric structure consisting of two anti-parallel heterodimers, each one formed of a large (p20) and a small (p10) subunit. Each heterodimer has a weight of 43kDa. Upon activation, murine caspase 11 is cleaved by an autocatalytic mechanism to give rise to the individual subunits. Expression levels of murine Caspase 11 are highest in the lung and spleen.

Alternate Names

CASP11, CASP-11, CASP4, CASP-4, caspase 4, apoptosis-related cysteine peptidase, Caspl, ich-3

Gene Symbol

Casp4

Additional Caspase-11 Products

Product Documents for Caspase-11 Antibody (17D9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Caspase-11 Antibody (17D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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