Caspase-8 Antibody (1G12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80097

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA, Flow Cytometry, Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 kappa Clone # 1G12

Format

BSA Free

Concentration

1 mg/ml

View all Caspase-8 Antibodies »

Product Specifications

Immunogen

Recombinant mouse caspase-8 (p20 subunit).

Specificity

Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1 kappa

Scientific Data Images for Caspase-8 Antibody (1G12) - BSA Free

Western Blot: Caspase-8 Antibody (1G12)BSA Free [NBP2-80097]

Western Blot: Caspase-8 Antibody (1G12)BSA Free [NBP2-80097]

Western Blot: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097] - Using Caspase-8 (mouse), mAb (1G12) (Prod. No. NBP2-80097) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.
Flow Cytometry: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097]

Flow Cytometry: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097]

Flow Cytometry: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097] - Data in permeabilized cells of overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) in 293T cells using Caspase-8 mAbs (1G12) (Prod. No. NBP2-80097)
Western Blot: Caspase-8 Antibody (1G12)BSA Free [NBP2-80097]

Western Blot: Caspase-8 Antibody (1G12)BSA Free [NBP2-80097]

Western Blot: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097] - Using Caspase-8 (mouse), mAb (1G12) (Prod. No. NBP2-80097) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.
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Applications for Caspase-8 Antibody (1G12) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-8

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. The sequential activation of caspases plays a central role in the execution-phase of cell apoptosis, and Caspase 8 is the most upstream protease in the the caspase activation cascade.

Caspase 8 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 8 form.

Caspase 8 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Alternate Names

CASP8, Caspase8, Mch5

Gene Symbol

CASP8

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody (1G12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Caspase-8 Antibody (1G12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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