Caspase-8 Antibody (1H11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22183

Novus Biologicals, part of Bio-Techne
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NBP2-22183
NBP2-22183-0.025ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H11

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Caspase-8 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Caspase-8 expressed in E. Coli.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-8 Antibody (1H11) - BSA Free

Western Blot: Caspase-8 Antibody (1H11)BSA Free [NBP2-22183]

Western Blot: Caspase-8 Antibody (1H11)BSA Free [NBP2-22183]

Western Blot: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis using Caspase 8 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), NIH/3T3 (4), Cos7 (5) and PC-12 (6) cell lysate.
Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) - BSA Free [NBP2-22183]

Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) - BSA Free [NBP2-22183]

Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis of liver cancer tissues using Caspase 8 mouse mAb with DAB staining.
Flow Cytometry: Caspase-8 Antibody (1H11) - BSA Free [NBP2-22183]

Flow Cytometry: Caspase-8 Antibody (1H11) - BSA Free [NBP2-22183]

Flow Cytometry: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis of NIH/3T3 cells using Caspase 8 mouse mAb (blue) and negative control (red).
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Applications for Caspase-8 Antibody (1H11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-8

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Alternate Names

CASP8, Caspase8, Mch5

Gene Symbol

CASP8

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody (1H11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Caspase-8 Antibody (1H11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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