May we ask if NB600-1343, NB100-56527 and NB500-208 can recognize both active form and profor

Product NB100-56527 recognises both pro and active forms of Caspase 8. NB500-208 recognises only the active form, and NB600-1343 has not been tested for specificity at this time.

Does NB500-208 both recognize human and rat active form of caspase 8 (subunit p18 or subunit p10)? Besides that please let me know if NB500-208 also recognize pro-form caspase 8?

Caspase 8 Antibody (FLICE 4-1-20) # NB500-208 antibody does not recognize rat samples. It is human specific and in our QC data it was successfully tested to detect the pro or inactive form of caspase at molecular weight 59kd. However, depending upon the extent of extrinsic apoptotic pathway activation in your cells or tissues, you can expect to see bands of the active form also.

May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?

In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Caspase-8 Antibody (FLICE 4-1-20)

Name: Caspase-8 Antibody (FLICE 4-1-20)
Brand: Novus Biologicals
SKU: NB500-208

Novus Biologicals, part of Bio-Techne | Catalog # NB500-208

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Product Datasheet / COA / SDS / Protocols & Manuals

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # FLICE 4-1-20

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Caspase-8 Antibodies »

Product Specifications

Immunogen

Full-length recombinant human Caspase 8 [UniProt# Q14790]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29069785).

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for Caspase-8 Antibody (FLICE 4-1-20)

Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208]

Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208] - CaSki cells were mock-infected or exposed to each of the indicated viruses (MOI ~ 1 pfu/cell) or 10 uM staurosporine and 4 h pi, cellular lysates were prepared and analyzed by immunoblotting for Caspase-8. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006766) licensed under a CC-BY license.

Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208]

Western Blot: Caspase 8 Antibody (FLICE 4-1-20) [NB500-208] - Detection of Caspase 8 (59 kDa) from HEK293 cell extract using NB500-208 (1:500). Lanes 1 and 2 contain inactive and active Caspase 8, respectively.

Applications for Caspase-8 Antibody (FLICE 4-1-20)

Application

Recommended Usage

Western Blot

1:1000

Application Notes

This Caspase 8 (FLICE 4-1-20) antibody is useful for Western Blot where a band is seen at 55 kDa representing the inactive form of Caspase 8 (see protocol for additional information). We also have reports that the antibody detects the active (pro) form of Caspase 8.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Caspase-8

Caspases are evolutionary conserved family of aspartate-specific cystein proteases which are essential part of apoptotic machinery and typically signal in a two-step cascade: 'initiator caspases' with large prodomains (such as caspase-1, -8 and -9) are first recruited into large protein complexes in which they undergo proximity-induced autoactivation and "executioner caspases" such as caspase-3 and -6, caspase-7 which are activated by initiator caspases via freeing of executioner caspases of their short inhibitory prodomain, allowing them to cleave a large set of cellular substrates. Localized in cytoplasm, caspase 7 is a heterotetramer that is consists of two anti-parallel arranged heterodimers, each one formed by a 20 kD (p20) and a 11 kD (p11) subunit, and interacts with BIRC6/bruce. Caspase 7 cleaves/activates SREBPs and poly(ADP-ribose) polymerase (PARP). Its overexpression promotes apoptosis, and it also implicates in inflammation signaling. Therapeutic intervention with caspase-7 has been shown to prove beneficial in conditions where excessive cell death and/or inflammation contribute to diseases e.g. Alzheimer's disease, Huntington's disease, sepsis, rheumatoid arthritis, cancer and NIDDM.

Alternate Names

CASP8, Caspase8, Mch5

Entrez Gene IDs

841 (Human)

Gene Symbol

CASP8

UniProt

Q14790

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody (FLICE 4-1-20)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download NB500-208 Specific Protocol

COA

SDS

Download SDS

Product Specific Notices for Caspase-8 Antibody (FLICE 4-1-20)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Caspase-8 Antibody (FLICE 4-1-20)

Novus Biologicals, part of Bio-Techne | Catalog # NB500-208

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all Caspase-8 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Clonality

Host

Isotype

Scientific Data Images for Caspase-8 Antibody (FLICE 4-1-20)

Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208]

Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208]

Applications for Caspase-8 Antibody (FLICE 4-1-20)

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Caspase-8

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody (FLICE 4-1-20)

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for Caspase-8 Antibody (FLICE 4-1-20)