Caspase-9 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76961

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Caspase-9 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 20 amino acids near the center of human Caspase 9. The immunogen is located within amino acids 290-340 of Caspase 9. Amino Acid Sequence: ASTSPEDESPGSNPEPDATP

Specificity

Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family. Human CASPASE 9 has 4 isoforms, including isoform 1 (416aa, 46kD), isoform 2 (266aa, 30kD), isoform 3 (154aa, 17kD), and isoform 4 (333aa, 37kD). Mouse CASPASE 9 has one isoform (454aa, 50kD) and Rat CASPASE 9 also has one isoform (454aa, 50kD). NBP1-76961 can detect human and mouse isoform.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-9 Antibody - BSA Free

Western Blot: Caspase-9 AntibodyBSA Free [NBP1-76961]

Western Blot: Caspase-9 AntibodyBSA Free [NBP1-76961]

Western Blot: Caspase-9 Antibody [NBP1-76961] - Western Blot Validation in Mouse Cell Line. Loading: 15 ug of 3T3/NIH cell lysate.Antibodies: : Caspase 9, NBP1-76961 (1 ug/mL)), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody - BSA Free [NBP1-76961]

Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody - BSA Free [NBP1-76961]

Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody [NBP1-76961] - Staining of Caspase-9 in HeLa cells with Caspase-9 antibody at 5 ug/ml.
Western Blot: Caspase-9 AntibodyBSA Free [NBP1-76961]

Western Blot: Caspase-9 AntibodyBSA Free [NBP1-76961]

Western Blot: Caspase-9 Antibody [NBP1-76961] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Caspase 9, NBP1-76470 (1 ug/mL), Caspase 9, NBP1-76961 (1 ug/mL) and beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Applications for Caspase-9 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry

2- 5 ug/mL

Immunocytochemistry/ Immunofluorescence

2-20 ug/mL

Immunofluorescence

5-20 ug/mL

Western Blot

1 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-9

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

Alternate Names

CASP9, Caspase9

Gene Symbol

CASP9

UniProt

P55211

Additional Caspase-9 Products

Product Documents for Caspase-9 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Caspase-9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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