CCL2/MCP1 Antibody (2D8) [Allophycocyanin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22115APC
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Feline, Primate
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 2D8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CCL2/MCP1 Antibody (2D8) [Allophycocyanin]
Immunogen
Purified recombinant fragment of human MCP1 expressed in E. coli.
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 29286133).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CCL2/MCP1 Antibody (2D8) [Allophycocyanin]
Flow Cytometry: CCL2/MCP1 Antibody (2D8) [Allophycocyanin] [NBP2-22115APC] - Viable CNS mononuclear cells isolated at onset (left) and peak (right) of EAE and stained for CD11b and CCL2. Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and mononuclear cells were isolated from the CNS at day 10 (onset of symptoms) and at day 15 (peak of disease). Cells were stained for CCL2, GFAP, Neun, CX3CL1, CXCL12, CD45 and CD11b, plus for viability to exclude dead cells. CCL2 staining is shown for viable cells (either CD11b+ or CD11b-) in samples isolated on day 10 (left) and 15 (right). Image from verified customer review.
Applications for CCL2/MCP1 Antibody (2D8) [Allophycocyanin]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NBP2-22115APC in the following applications:
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CCL2/MCP1
Alternate Names
C-C motif chemokine 2, chemokine (C-C motif) ligand 2, GDCF-2, HC11Monocyte secretory protein JE, MCAFMonocyte chemotactic and activating factor, MCP1 Monocyte chemotactic protein 1, MCP-1 Small-inducible cytokine A2, MGC9434, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, SCYA2HSMCR30, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je), small inducible cytokine subfamily A (Cys-Cys), member 2, SMC-CF
Gene Symbol
CCL2
Additional CCL2/MCP1 Products
Product Documents for CCL2/MCP1 Antibody (2D8) [Allophycocyanin]
Product Specific Notices for CCL2/MCP1 Antibody (2D8) [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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