CCL2/MCP1 Antibody (2D8) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80618
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat, Feline, Primate
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2D8
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Full lenght human MCP1 expressed in E. coli.
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 29286133).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CCL2/MCP1 Antibody (2D8) - Azide and BSA Free
Western Blot: CCL2/MCP1 Antibody (2D8)Azide and BSA Free [NBP2-80618]
Western Blot: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis using MCP1 mouse mAb against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6) cell lysates. Image from the standard format of this antibody.Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618]
Immunocytochemistry/Immunofluorescence: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - CCL2 staining in acini cultures from MMTV-HER2 MGs (20-24 weeks) grown for 5 days and then DMSO-treated (vehicle control, e), 1uM lapatinib (f) or 1 uM IKKi compound A (g) for 24 hrs. Bar: 25 um. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-017-02481-5), licensed under a CC-BY license. Image from the standard format of this antibody.Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618]
Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis of paraffin-embedded liver cancer tissues using MCP1 mouse mAb with DAB staining. Image from the standard format of this antibody.Applications for CCL2/MCP1 Antibody (2D8) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CCL2/MCP1
Alternate Names
C-C motif chemokine 2, chemokine (C-C motif) ligand 2, GDCF-2, HC11Monocyte secretory protein JE, MCAFMonocyte chemotactic and activating factor, MCP1 Monocyte chemotactic protein 1, MCP-1 Small-inducible cytokine A2, MGC9434, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, SCYA2HSMCR30, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je), small inducible cytokine subfamily A (Cys-Cys), member 2, SMC-CF
Gene Symbol
CCL2
Additional CCL2/MCP1 Products
Product Documents for CCL2/MCP1 Antibody (2D8) - Azide and BSA Free
Product Specific Notices for CCL2/MCP1 Antibody (2D8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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