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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Rat IgG1 Clone # 114906

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL20/MIP-3 alpha
Ala28-Met97
Accession # O89093

Specificity

Detects mouse CCL20/MIP‑3 alpha in direct ELISAs and Western blots. In direct ELISAs, 12% cross-reactivity with recombinant rat (rr) CCL20, 6% cross-reactivity with recombinant mouse (rm) CCL12 and recombinant human (rh) CCL25, and 3% cross‑reactivity with with rmCCL9/10/MIP-1 gamma is observed. No cross-reactivity with recombinant cotton rat CCL3, 4, 5, rhCCL1, 2, 3, 4, 5, 7, 8, 11 ,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26, 27, 28, rmCCL1, 2, 3, 4, 5, 6, 7, 8, 11, 17, 19, 21, 22, 24, 25, 27, or 28 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for CCL20/MIP-3 alpha Antibody (114906) [CoraFluor™ 1]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CCL20/MIP-3 alpha

Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibitsproliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of themucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processedforms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coliATCC 25922 and S.aureus ATCC 29213

Alternate Names

exodus-1, LARC, MIP-3 alpha, MIP3 alpha

Gene Symbol

CCL20

Additional CCL20/MIP-3 alpha Products

Product Documents for CCL20/MIP-3 alpha Antibody (114906) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CCL20/MIP-3 alpha Antibody (114906) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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