Detects mouse CCL20/MIP‑3 alpha in direct ELISAs and Western blots. In direct ELISAs, 12% cross-reactivity with recombinant rat (rr) CCL20, 6% cross-reactivity with recombinant mouse (rm) CCL12 and recombinant human (rh) CCL25, and 3% cross‑reactivity with with rmCCL9/10/MIP-1 gamma is observed. No cross-reactivity with recombinant cotton rat CCL3, 4, 5, rhCCL1, 2, 3, 4, 5, 7, 8, 11 ,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26, 27, 28, rmCCL1, 2, 3, 4, 5, 6, 7, 8, 11, 17, 19, 21, 22, 24, 25, 27, or 28 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Scientific Data Images for Mouse CCL20/MIP-3 alpha Antibody

Detection of Mouse CCL20 by Western Blot.

Western blot shows lysates of mouse small intestine. PVDF membrane was probed with 2 µg/mL of Mouse CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB760) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCL20 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse CCL20/MIP-3 alpha by Western Blot

Conditional macrophage ablation disrupted Treg-cell recruitment and inhibited the growth of CRC in mice.(A, B) Flow cytometric analysis of TAMs derived from grafted CRC in CD11b-DTR mice injected with DTmu or DT. (C) CCL20 mRNA levels in grafted CRC in CD11b-DTR mice injected with DTmu or DT (data from 5 mice of each group were pooled together). (D) Lysates of grafted CRC from CD11b-DTR mice injected with DTmu or DT were subjected to western blotting for CCL20 expression. Actin - loading control. (E) Immunofluorescence staining with anti-mouse CCL20 was performed on cryosections of GFP+ CMT93 grafted tumor mass derived from CD11b-DTR mice injected with DTmu or DT for 14 days. (F) Flow cytometric analysis of CCR6 expression for GFP+ CMT93 cells before grafting (left panel), and CCR6 staining on cryosections of GFP+ CMT93 grafted tumor mass derived from CD11b-DTR mice injected with DTmu or DT for 14 days (middle and right panel). (G, H) Number of tumor-infiltrating FoxP3+ Treg-cells in grafted CRC in CD11b-DTR mice injected with DTmu or DT. (I) CRC grafted in CD11b-DTR mice injected with DTmu or DT for 14 days. (J) Tumor weight of CRC grafted in CD11b-DTR mice injected with DTmu or DT for 14 days. Representative data are shown which had been reproduced in 2 independent experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0019495), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CCL20/MIP-3 alpha Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: Mouse small intestine

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB760 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL20/MIP-3 alpha

CCL20, also known as MIP-3 alpha, LARC, and Exodus, is a beta chemokine. Mouse CCL20 cDNA encodes a 97 amino acid precursor protein with a 27 aa putative signal peptide that is predicted to be cleaved to form the 70 aa mature secreted protein. CCL20 is distantly related to other beta chemokines (20‑28% aa sequence identity). Mouse CCL20 shares approximately 71 and 63% amino acid sequence homology with rat and human CCL20, respectively. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of CCL20 is strongly upregulated by inflammatory signals and downregulated by the anti-inflammatory cytokine IL-10. CCL20 has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. CCL20 is a functional ligand for CCR6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34⁺ cord blood precursors.

References

Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
Hromas, R. et al. (1997) Blood 89:3315.
Greaves, D. R. et al. (1997) J. Exp. Med. 186: 857.
Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.

Alternate Names

exodus-1, LARC, MIP-3 alpha, MIP3 alpha

Entrez Gene IDs

6364 (Human); 20297 (Mouse); 29538 (Rat)

Gene Symbol

CCL20

UniProt

O89093

Additional CCL20/MIP-3 alpha Products

Product Documents for Mouse CCL20/MIP-3 alpha Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse CCL20/MIP-3 alpha Antibody

For research use only

Citations
Reviews

Mouse CCL20/MIP-3 alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB760

Key Product Details

Species Reactivity

Validated:

Cited:

Applications