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CCR2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48338

Novus Biologicals, part of Bio-Techne
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NBP1-48338
NBP1-48338SS

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Porcine

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen

Cited:

Block/Neutralize, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide made to an N-terminal portion of the mouse CCR2 protein (within residues 20-100). [Swiss-Prot# P51683]

Localization

Cell membrane; Multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCR2 Antibody - BSA Free

Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48338]

Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48338]

Flow Cytometry: CCR2 Antibody [NBP1-48338] - An intracellular stain was performed on THP-1 cells with CCR2 Antibody NBP1-48338G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.
Immunocytochemistry/ Immunofluorescence: CCR2 Antibody - BSA Free [NBP1-48338]

Immunocytochemistry/ Immunofluorescence: CCR2 Antibody - BSA Free [NBP1-48338]

Immunocytochemistry/Immunofluorescence: CCR2 Antibody [NBP1-48338] - Analysis of CCR2 in NIH/3T3 cells.
Immunohistochemistry: CCR2 Antibody - BSA Free [NBP1-48338]

Immunohistochemistry: CCR2 Antibody - BSA Free [NBP1-48338]

Immunohistochemistry: CCR2 Antibody [NBP1-48338] - Expression patterns of CCL2 and CCR2 in unwounded and wounded corneas of WT and MMP12 KO mice. Immunofluorescence of CCL2 chemokine receptor CCR2 in unwounded and chemically wounded (2-days after injury) WT and Mmp12-/- mouse corneas. Control images represent mouse corneas stained with secondary antibody only and without primary antibody. Nuclei were visualized by staining with DAPI (blue). Scale bars: 50 um. CCR2 staining was visualized in epithelial and stromal layers of unwounded and wounded WT and Mmp12-/- corneas. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-019-47831-z), licensed under a CC-BY license.

Applications for CCR2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:200

Immunohistochemistry-Frozen

reported in scientific literature (PMID 31399604)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CCR2

CCR2 is a major chemokine receptor expressed on monocytes and its activation through its ligands MCP1, MCP2, MCP3 and MCP4 (systematic names CCL2, CCL8, CCL7 and CCL13, respectively) stimulates the migration of monocytes across the vascular wall into tissues wherein they facilitate chronic inflammation. CCR2 is a member of GPCR1 family and is up-regulated by CREB3. It is a multi-pass membrane protein found in cell membrane, and besides monocytes, it is also found on other cell types like NK cells, macrophages, immature dendritic cells, gamma delta T cells, and activated T cells (including Th17 cells). There are two alternatively spliced forms of CCR2: CCR2A - the major isoform expressed by mononuclear cells and vascular smooth muscle cells; and CCR2B - expressed predominantly by monocytes and activated NK cells. CCR2 has both pro-inflammatory (mediated by APC and T cells) and anti-inflammatory (mediated by regulatory T cells) activities. Besides inflammation, it has been implicated several pathologies including HIV-1/AIDS, autoimmune disorders (psoriasis, rheumatoid arthritis, and multiple sclerosis), pulmonary diseases (asthma, COPD), vascular disease and cancer (prostate cancer).

Alternate Names

CC-CKR-2, CCR2, CCR2B, CD192, CKR2, CKR2A, CMKBR2, FLJ78302, MCP-1-R

Entrez Gene IDs

12772 (Mouse)

Gene Symbol

CCR2

UniProt

Additional CCR2 Products

Product Documents for CCR2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CCR2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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