CCR2 was detected in immersion fixed NIH3T3 Mouse fibroblast cell line using Rabbit anti-CCR2 Antigen Affinity-purified Polyclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-48338R) (red) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for CCR2 Antibody [DyLight 550]

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CCR2

CCR2, a Chemokine Receptor, is a regulator of monocyte/macrophage traffickings and is upregulated in response to inflammation. Resistance to HIV-1 infection or delayed progression to AIDS may be linked to CCR2 polymorphisms. The ligand for CCR2 is monocyte chemoattractant protein (MCP-1), a chemokine produced by endothelial cells, smooth muscle cells, and macrophages. Activation of monocytes by MCP-1 may mediate the monocytic infiltration of tissues in atherosclerosis (Boring et al., 1998) and other inflammatory disorders including rheumatoid arthritis, alveolitis, and tumor infiltration. Two isoforms are produced by alternative splicing. CCR2 has been reported to be expressed in a wide variety of tissues, including blood, brain, heart, kidney, liver, lung, ovary, pancreas, spinal cord, spleen, thymus, and vessel. Increased expression of CCR2 has been documented in CD3+ lymphocytes in rheumatoid arthritis (Ruth et al., 2001). ESTs have been isolated from liver/spleen and prostate libraries.

Alternate Names

CC-CKR-2, CCR2, CCR2B, CD192, CKR2, CKR2A, CMKBR2, FLJ78302, MCP-1-R

Gene Symbol

CCR2

Additional CCR2 Products

Product Documents for CCR2 Antibody [DyLight 550]

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CCR2 Antibody [DyLight 550]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Spectra Viewer
Flow Cytometry

CCR2 Antibody [DyLight 550]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48338R

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all CCR2 Antibodies »

Product Specifications

Immunogen

Localization

Clonality

Host

Isotype

Scientific Data Images for CCR2 Antibody [DyLight 550]

CCR2 in NIH-3T3 Mouse Cell Line.

Applications for CCR2 Antibody [DyLight 550]

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Frozen

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: CCR2

Alternate Names

Gene Symbol

Additional CCR2 Products

Product Documents for CCR2 Antibody [DyLight 550]

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CCR2 Antibody [DyLight 550]