CD117/c-kit Antibody (C117/8879R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23881
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # C117/8879R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 1-200) of human CD117/c-kit protein (exact sequence is proprietary)
Localization
Cell surface. Cytoplasm.
Specificity
This monoclonal antibody recognizes a protein of 145kDa, identified as CD117/p145kit. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
Marker
Marker for Gastrointestinal Stromal Tumors
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Store at 2 to 8C. Non-hazardous. No MSDS required.
Scientific Data Images for CD117/c-kit Antibody (C117/8879R)
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (C117/8879R) [NBP3-23881] -
Formalin-fixed, paraffin-embedded human GIST stained with CD117/c-kit antibody (C117/8879R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for CD117/c-kit Antibody (C117/8879R)
Application
Recommended Usage
ELISA
2-4ug/ml
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
ELISA: For coating, order antibody without BSA.
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD117/c-kit
Long Name
Stem Cell Factor Receptor
Alternate Names
c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR
Gene Symbol
KIT
Additional CD117/c-kit Products
Product Documents for CD117/c-kit Antibody (C117/8879R)
Product Specific Notices for CD117/c-kit Antibody (C117/8879R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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