CD117/c-kit Antibody (C117/8879R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24160
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Kappa Clone # C117/8879R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 1-200) of human CD117/c-kit protein (exact sequence is proprietary)
Localization
Cell surface. Cytoplasm.
Specificity
This monoclonal antibody recognizes a protein of 145kDa, identified as CD117/p145kit. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
Marker
Marker for Gastrointestinal Stromal Tumors
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD117/c-kit Antibody (C117/8879R) - Azide and BSA Free
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (C117/8879R) - Azide and BSA Free [NBP3-24160] -
Formalin-fixed, paraffin-embedded human GIST stained with CD117/c-kit antibody (C117/8879R) - Azide and BSA Free at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for CD117/c-kit Antibody (C117/8879R) - Azide and BSA Free
Application
Recommended Usage
ELISA
2-4ug/ml
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
ELISA: For coating, order antibody without BSA.
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD117/c-kit
Long Name
Stem Cell Factor Receptor
Alternate Names
c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR
Gene Symbol
KIT
Additional CD117/c-kit Products
Product Documents for CD117/c-kit Antibody (C117/8879R) - Azide and BSA Free
Product Specific Notices for CD117/c-kit Antibody (C117/8879R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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