CD11b Antibody (OX-42) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65284
![Flow Cytometry: CD11b Antibody (OX-42) - BSA Free [NB100-65284]](https://resources.bio-techne.com/images/products/CD11b-Antibody-OX-42-Flow-Cytometry-NB100-65284-img0001.jpg "Flow Cytometry: CD11b Antibody (OX-42) - BSA Free [NB100-65284]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Bovine
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OX-42
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Mouse Monoclonal CD11b Antibody (OX-42) was made from rat peritoneal macrophages.
Specificity
NB100-65284 recognizes the rat equivalent of human CD11b, the receptor for the iC3b component of complement. The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. This CD11b antibody also labels dendritic cells, granulocytes and microglial cells in the brain. Clone MRC OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Functionally, clone OX-42 inhibits complement mediated rosettes.
Marker
Microglia Marker, Macrophage Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
127.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CD11b Antibody (OX-42) - BSA Free
Flow Cytometry: CD11b Antibody (OX-42) - BSA Free [NB100-65284]
Flow Cytometry: CD11b Antibody (OX-42) [NB100-65284] - Detection of CD11b in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Rat CD11b Monoclonal Antibody (OX-42) [NB100-65284, filled histogram] or Mouse IgG2A Isotype Control Antibody [MAB003, open histogram] followed by anti-Mouse IgG PE-conjugated secondary antibody [F0102B] and Mouse Anti-Rat CD161 Alexa Fluor 647-conjugated Monoclonal Antibody [FAB9766R]. Splenocytes were gated on CD161+ cells.
Flow Cytometry: CD11b Antibody (OX-42) [NB100-65284]
Flow Cytometry: CD11b Antibody (OX-42) [NB100-65284] - Detection of CD11b in Rat Bone Marrow cells by Flow Cytometry. Rat cells were stained with Mouse Anti-Rat CD11b Monoclonal Antibody (OX-42) [NB100-65284, filled histogram] or Mouse IgG2A Isotype Control Antibody [MAB003, open histogram] followed by anti-Mouse IgG APC-conjugated secondary antibody [F0101B] .Applications for CD11b Antibody (OX-42) - BSA Free
Application
Recommended Usage
Immunohistochemistry-Frozen
1:50 - 1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD11b/Integrin alpha M
References
1. Rosetti, F., & Mayadas, T. N. (2016). The many faces of Mac-1 in autoimmune disease. Immunol Rev, 269(1), 175-193. doi:10.1111/imr.12373
2. Kim, D., Kim, T. H., Wu, G., Park, B. K., Ha, J. H., Kim, Y. S., . . . Kwon, H. J. (2016). Extracellular Release of CD11b by TLR9 Stimulation in Macrophages. PLoS One, 11(3), e0150677. doi:10.1371/journal.pone.0150677
3. Christensen, J. E., Andreasen, S. O., Christensen, J. P., & Thomsen, A. R. (2001). CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. Int Immunol, 13(4), 593-600. doi:10.1093/intimm/13.4.593
4. Nath, S. K., Han, S., Kim-Howard, X., Kelly, J. A., Viswanathan, P., Gilkeson, G. S., . . . Harley, J. B. (2008). A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus. Nat Genet, 40(2), 152-154. doi:10.1038/ng.71
Alternate Names
CD11b, Integrin alpha M, ITGAM, cd11b flow antibody, M1/70, M1/70 cd11b, m1/70 clone, M1/70.15 Alexa Fluor, M1/70.15 Antibody, M1/70.15 CD11b, M1/70.15 CD11b Antibody, M1/70.15 Clone, M1/70.15 Conjugated, M1/70.15 ICC Antibody, M1/70.15 IHC Antibody, M1/70.15 Immunocytochemistry Antibody, M1/70.15 Immunohistochemistry Antibody, M1/70.15 Microglia Marker, M1/70.15 Myeloid Marker, MAC 1, MAC1, Mac1, alpha subunit, Mac-1a, Mo1, alpha subunit
Entrez Gene IDs
25021 (Rat)
Gene Symbol
ITGAM
Additional CD11b/Integrin alpha M Products
Product Documents for CD11b Antibody (OX-42) - BSA Free
Product Specific Notices for CD11b Antibody (OX-42) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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