CD11b Antibody (OX-42) [mFluor Violet 610 SE]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65284MFV610
![CD11b Antibody (OX-42) [mFluor Violet 610 SE]](https://resources.bio-techne.com/images/products/nb100-65284mfv610_mouse-cd11b-mab-ox-42-mfluor-violet-610-se-6720239102523.png "CD11b Antibody (OX-42) [mFluor Violet 610 SE] [NB100-65284MFV610] -")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
mFluor Violet 610 SE (Excitation = 421 nm, Emission = 613 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # OX-42
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CD11b Antibody (OX-42) [mFluor Violet 610 SE]
Immunogen
Mouse Monoclonal CD11b Antibody (OX-42) was made from rat peritoneal macrophages.
Specificity
NB100-65284 recognizes the rat equivalent of human CD11b, the receptor for the iC3b component of complement. The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. This CD11b antibody also labels dendritic cells, granulocytes and microglial cells in the brain. Clone MRC OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Functionally, clone OX-42 inhibits complement mediated rosettes.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for CD11b Antibody (OX-42) [mFluor Violet 610 SE]
CD11b Antibody (OX-42) [mFluor Violet 610 SE] [NB100-65284MFV610] -
CD11b Antibody (OX-42) [mFluor Violet 610 SE] [NB100-65284MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission maximum of 613 nm.Applications for CD11b Antibody (OX-42) [mFluor Violet 610 SE]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CD11b/Integrin alpha M
References
1. Rosetti, F., & Mayadas, T. N. (2016). The many faces of Mac-1 in autoimmune disease. Immunol Rev, 269(1), 175-193. doi:10.1111/imr.12373
2. Kim, D., Kim, T. H., Wu, G., Park, B. K., Ha, J. H., Kim, Y. S., . . . Kwon, H. J. (2016). Extracellular Release of CD11b by TLR9 Stimulation in Macrophages. PLoS One, 11(3), e0150677. doi:10.1371/journal.pone.0150677
3. Christensen, J. E., Andreasen, S. O., Christensen, J. P., & Thomsen, A. R. (2001). CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. Int Immunol, 13(4), 593-600. doi:10.1093/intimm/13.4.593
4. Nath, S. K., Han, S., Kim-Howard, X., Kelly, J. A., Viswanathan, P., Gilkeson, G. S., . . . Harley, J. B. (2008). A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus. Nat Genet, 40(2), 152-154. doi:10.1038/ng.71
Alternate Names
CD11b, Integrin alpha M, ITGAM
Gene Symbol
ITGAM
Additional CD11b/Integrin alpha M Products
Product Documents for CD11b Antibody (OX-42) [mFluor Violet 610 SE]
Product Specific Notices for CD11b Antibody (OX-42) [mFluor Violet 610 SE]
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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