CD14 Antibody (M5E2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-77758

Clone M5E2 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bovine, Canine, Primate

Cited:

Human, Porcine, Canine

Applications

Validated:

Block/Neutralize, CyTOF-ready, Dual RNAscope ISH-IHC, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Simple Western

Cited:

Block/Neutralize, Flow Cytometry, IF/IHC, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # M5E2

Format

BSA Free

Concentration

1.0 mg/ml

View all CD14 Antibodies »

Product Specifications

Immunogen

Human peripheral blood cells.

Reactivity Notes

Expected to cross react with Chimpanzee, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Cotton-topped Tamarin, Common Marmoset, Squirrel Monkey.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CD14 Antibody (M5E2) - BSA Free

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (M5E2) - BSA Free [NB100-77758]

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (M5E2) - BSA Free [NB100-77758]

Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) [NB100-77758] - Representative immunostaining of CD14 (red) in adult human lung tissue sample with nuclei counterstained blue. This image was submitted by customer review. Image from the Alexa Fluor 647 version of this antibody.
Flow Cytometry: CD14 Antibody (M5E2) - BSA Free [NB100-77758]

Flow Cytometry: CD14 Antibody (M5E2) - BSA Free [NB100-77758]

Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - A surface stain was performed on human peripheral blood monocytes with CD163 (EDHu-1) antibody NB110-40686APC and a matched isotype control NBP2-27287APC. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488.
Immunocytochemistry/ Immunofluorescence: CD14 Antibody (M5E2) - BSA Free [NB100-77758]

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (M5E2) - BSA Free [NB100-77758]

Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) [NB100-77758] - Human lung tissue was stained with FITC-conjugated CD14 (Green) for overnight. Image from the Alexa Fluor 488 version of this antibody.
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Applications for CD14 Antibody (M5E2) - BSA Free

Application
Recommended Usage

Block/Neutralize

reported in scientific literature (PMID 27228163)

Flow Cytometry

< 2.0 ug/10^6 cells in 100 ul

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Simple Western

50 ug/ml
Application Notes
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation, and immunofluorescence microscopy. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. This antibody is CyTOF ready. See Simple Western Antibody Database for Simple Western validation: tested in THP-1; antibody dilution of 50 ug/ml; separated by size; detects a band at 54 kDa

Reviewed Applications

Read 2 reviews rated 3 using NB100-77758 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD14

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.

Alternate Names

CD14

Entrez Gene IDs

929 (Human)

Gene Symbol

CD14

Additional CD14 Products

Product Documents for CD14 Antibody (M5E2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD14 Antibody (M5E2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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