CD14 Antibody (M5E2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80627

Clone M5E2 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bovine, Canine, Primate

Applications

Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # M5E2

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD14 Antibodies »

Product Summary for CD14 Antibody (M5E2) - Azide and BSA Free

Immunogen

Human peripheral blood cells.

Reactivity Notes

Expected to cross react with Chimpanzee, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Cotton-topped Tamarin, Common Marmoset, Squirrel Monkey.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CD14 Antibody (M5E2) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627]

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627]

Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Representative immunostaining of CD14 (red) in adult human lung tissue sample with nuclei counterstained blue. This image was submitted by customer review. Image from the Alexa Fluor 647 version of this antibody. Image from the standard format of this antibody.
Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627]

Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627]

Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Analysis using the PE conjugate of NB100-77758. Staining of CD14 in human PBMC using anti-CD14 antibody conjugated with PE. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627]

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627]

Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Human lung tissue was stained with FITC-conjugated CD14 (Green) for overnight. Image from the Alexa Fluor 488 version of this antibody.
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Applications for CD14 Antibody (M5E2) - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

reported in scientific literature (PMID 27228163)

Flow Cytometry

2.0 ug/10^6 cells in 100 ul

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500
Application Notes
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation, and immunofluorescence microscopy. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD14

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.

Alternate Names

CD14

Gene Symbol

CD14

Additional CD14 Products

Product Documents for CD14 Antibody (M5E2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD14 Antibody (M5E2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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