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CD27 Ligand/TNFSF7/CD70 Antibody (TNFS7/1026) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11587

Novus Biologicals, part of Bio-Techne
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NBP3-11587

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # TNFS7/1026

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant human CD27 Ligand/TNFSF7/CD70 protein (Uniprot: P32970)

Localization

Cell Surface

Marker

Activated T- & B-Lymphocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44546).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for CD27 Ligand/TNFSF7/CD70 Antibody (TNFS7/1026) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Frozen

0.5 - 1 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD27 Ligand/TNFSF7

CD70 is a type II transmembrane protein. It is a member of the TNF superfamily, expressed on activated B cells, activated dendritic cells, and some activated T cells (low levels). CD70 interacts with CD27 to promote T-B cell cross-stimulation and co-stimulate B cell proliferation and immunoglobulin production. Cells expressing CD70 can co-stimulate T cell proliferation and enhance the production of cytokines. The FR70 antibody blocks CD70 binding to CD27.

Alternate Names

CD70, TNFSF7

Gene Symbol

CD70

Additional CD27 Ligand/TNFSF7 Products

Product Documents for CD27 Ligand/TNFSF7/CD70 Antibody (TNFS7/1026) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD27 Ligand/TNFSF7/CD70 Antibody (TNFS7/1026) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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