CD3 epsilon Antibody (UCHT1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2726

Novus Biologicals, part of Bio-Techne
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NB100-2726
NB100-2726SS
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Key Product Details

Species Reactivity

Human, Porcine, Primate

Applications

Dual RNAscope ISH-IHC, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Radioimmunodiffusion, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # UCHT1

Format

BSA Free

Concentration

1.0 mg/ml

View all CD3 epsilon Antibodies »

Product Specifications

Immunogen

Human infant thymocytes and Sezary cells.

Epitope

Epsilon-chain

Localization

Type I membrane protein.

Specificity

Detects the non-glycosylated epsilon chain of CD3 (MW 20kD) which is closely associated on the lymphocyte cell surface with the T cell antigen receptor (TCR).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CD3 epsilon Antibody (UCHT1) - BSA Free

Western Blot: CD3 epsilon Antibody (UCHT1)BSA Free [NB100-2726]

Western Blot: CD3 epsilon Antibody (UCHT1)BSA Free [NB100-2726]

Western Blot: CD3 epsilon Antibody (UCHT1) [NB100-2726] - Western blot shows cell lysates MOLT-4, Jurket, Raji, and THP-1. Membrane was probed with 2 ug/mL of Mouse Anti-CD3 epsilon Monoclonal Antibody (Catalog # NB100-2726) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD3 epsilon at approximately 21 kDa (as indicated) in the first 2 cell lysates but was absent from the later 2. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.
Flow Cytometry: CD3 epsilon Antibody (UCHT1) - BSA Free [NB100-2726]

Flow Cytometry: CD3 epsilon Antibody (UCHT1) - BSA Free [NB100-2726]

Flow Cytometry: CD3 epsilon Antibody (UCHT1) [NB100-2726] - Analysis of PE/Cy5 conjugate of NB100-2726. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy5 (green) or Anti-Human CD3 PE-Cy5 (blue). Cells in the lymphocyte gate were used for analysis.
Flow Cytometry: CD3 epsilon Antibody (UCHT1) - BSA Free [NB100-2726]

Flow Cytometry: CD3 epsilon Antibody (UCHT1) - BSA Free [NB100-2726]

Flow Cytometry: CD3 epsilon Antibody (UCHT1) [NB100-2726] - Staining of human peripheral blood lymphocytes with Mouse anti-Human CD3: RPE.
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Applications for CD3 epsilon Antibody (UCHT1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 epsilon

CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Alternate Names

CD3e

Entrez Gene IDs

916 (Human); 917 (Human); 915 (Human)

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (UCHT1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD3 epsilon Antibody (UCHT1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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