CD3 epsilon Antibody (UCHT1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80638

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-80638
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Conjugate
Catalog #
Alexa Fluor 350
NB100-2726AF350
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Alexa Fluor 405
NB100-2726AF405
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Alexa Fluor 488
NB100-2726AF488
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Alexa Fluor 532
NB100-2726AF532
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Alexa Fluor 594
NB100-2726AF594
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Alexa Fluor 647
NB100-2726AF647
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Alexa Fluor 700
NB100-2726AF700
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Alexa Fluor 750
NB100-2726AF750
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Allophycocyanin
NB100-2726APC
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Allophycocyanin/Cy7
NB100-2726APCCY7
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Biotin
NB100-2726B
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CoraFluor 1
NB100-2726CL1
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DyLight 350
NB100-2726UV
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DyLight 405
NB100-2726V
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DyLight 488
NB100-2726G
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DyLight 550
NB100-2726R
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DyLight 594
NB100-2726DL594
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DyLight 650
NB100-2726C
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DyLight 680
NB100-2726FR
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DyLight 755
NB100-2726IR
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FITC
NB100-2726F
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HRP
NB100-2726H
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Janelia Fluor 525
NB100-2726JF525
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Janelia Fluor 549
NB100-2726JF549
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Janelia Fluor 585
NB100-2726JF585
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Janelia Fluor 635
NB100-2726JF635
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Janelia Fluor 646
NB100-2726JF646
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Janelia Fluor 669
NB100-2726JF669
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mFluor Violet 450 SE
NB100-2726MFV450
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mFluor Violet 500 SE
NB100-2726MFV500
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mFluor Violet 610 SE
NB100-2726MFV610
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PE
NB100-2726PE
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PE/Atto594
NB100-2726PEATT594
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PE/Cy5.5
NB100-2726PECY55
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PE/Cy7
NB100-2726PECY7
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PerCP
NB100-2726PCP
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Forumulation
Catalog #
BSA Free
NB100-2726
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Radioimmunodiffusion

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # UCHT1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD3 epsilon Antibodies »

Product Summary for CD3 epsilon Antibody (UCHT1) - Azide and BSA Free

Immunogen

Human infant thymocytes and Sezary cells.

Epitope

Epsilon-chain

Localization

Type I membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CD3 epsilon Antibody (UCHT1) - Azide and BSA Free

Flow Cytometry: CD3 epsilon Antibody (UCHT1) - Azide and BSA Free [NBP2-80638]

Flow Cytometry: CD3 epsilon Antibody (UCHT1) - Azide and BSA Free [NBP2-80638]

Flow Cytometry: CD3 epsilon Antibody (UCHT1) - Azide and BSA Free [NBP2-80638] - Staining of human peripheral blood lymphocytes with Mouse anti-Human CD3: RPE. Image from the standard format of this antibody.
Flow Cytometry: CD3 epsilon Antibody (UCHT1) - Azide and BSA Free [NBP2-80638]

Flow Cytometry: CD3 epsilon Antibody (UCHT1) - Azide and BSA Free [NBP2-80638]

Flow Cytometry: CD3 epsilon Antibody (UCHT1) - Azide and BSA Free [NBP2-80638] - Analysis of PE/Cy5 conjugate of NB100-2726. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy5 (green) or Anti-Human CD3 PE-Cy5 (blue). Cells in the lymphocyte gate were used for analysis.

Applications for CD3 epsilon Antibody (UCHT1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 epsilon

CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Alternate Names

CD3e

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (UCHT1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD3 epsilon Antibody (UCHT1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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