CD3 zeta [p Tyr142] Antibody (EM-54) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37708

Novus Biologicals, part of Bio-Techne
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NBP2-37708
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Flow (Intracellular), Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # EM-54

Format

BSA Free

Concentration

1 mg/ml

View all CD3 zeta/CD247 Antibodies »

Product Summary for CD3 zeta [p Tyr142] Antibody (EM-54) - BSA Free

Immunogen

CP1 (pTyr142) - KLH.

Modification

p Tyr142

Specificity

Antibody recognizes phosphorylated tyrosine 142 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD3 zeta [p Tyr142] Antibody (EM-54) - BSA Free

Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5

Applications for CD3 zeta [p Tyr142] Antibody (EM-54) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1-4 ug/ml

Flow Cytometry

1-4 ug/ml

Western Blot

1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD3 zeta/CD247

CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C. CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Loss of the zeta chain results in the synthesis of unstable TCRs. A decrease of CD3 zeta has been described in T cells from patients with cancer, lupus and chronic infectious diseases.

Long Name

T-cell Receptor zeta

Alternate Names

CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain

Gene Symbol

CD247

Additional CD3 zeta/CD247 Products

Product Documents for CD3 zeta [p Tyr142] Antibody (EM-54) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD3 zeta [p Tyr142] Antibody (EM-54) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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