CD3 zeta [p Tyr142] Antibody (EM-54) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37708CL1
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Flow (Intracellular), Flow Cytometry, Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # EM-54
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CD3 zeta [p Tyr142] Antibody (EM-54) [CoraFluor™ 1]
Immunogen
CP1 (pTyr142) - KLH.
Modification
p Tyr142
Specificity
Antibody recognizes phosphorylated tyrosine 142 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for CD3 zeta [p Tyr142] Antibody (EM-54) [CoraFluor™ 1]
Application
Recommended Usage
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: CD3 zeta/CD247
Long Name
T-cell Receptor zeta
Alternate Names
CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain
Gene Symbol
CD247
Additional CD3 zeta/CD247 Products
Product Documents for CD3 zeta [p Tyr142] Antibody (EM-54) [CoraFluor™ 1]
Product Specific Notices for CD3 zeta [p Tyr142] Antibody (EM-54) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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