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CD40 Ligand/TNFSF5 Antibody (chi220) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12025CL1

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Block/Neutralize, Immunocytochemistry/ Immunofluorescence

Label

CoraFluor 1

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # chi220

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for CD40 Ligand/TNFSF5 Antibody (chi220) [CoraFluor™ 1]

Immunogen

This antibody was generated by immunizing female BALB/c mice with a fusion protein containing extracellular domain of human CD40 Ligand/TNFSF5 fused to the hinge CH2 and CH3 domains of a murine IgG2b antibody.

Specificity

This antibody binds the CD40 Ligand/TNFSF5 and blocks the humoral immune responses to T cell dependent antigens in a dose dependent manner.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for CD40 Ligand/TNFSF5 Antibody (chi220) [CoraFluor™ 1]

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CD40 Ligand/TNFSF5

CD40L is the ligand for CD40, a member of the tumor necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L.

Alternate Names

CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP

Gene Symbol

CD40LG

Additional CD40 Ligand/TNFSF5 Products

Product Documents for CD40 Ligand/TNFSF5 Antibody (chi220) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD40 Ligand/TNFSF5 Antibody (chi220) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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