CD40/TNFRSF5 Antibody (C40/1605)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53389

Novus Biologicals, part of Bio-Techne
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NBP2-53389-100ug
NBP2-53389-20ug
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Conjugate
Catalog #
Alexa Fluor 350
NBP2-54502AF350
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Alexa Fluor 405
NBP2-54502AF405
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Alexa Fluor 488
NBP2-54502AF488
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Alexa Fluor 532
NBP2-54502AF532
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Alexa Fluor 594
NBP2-54502AF594
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Alexa Fluor 647
NBP2-54502AF647
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Alexa Fluor 700
NBP2-54502AF700
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Alexa Fluor 750
NBP2-54502AF750
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Allophycocyanin
NBP2-54502APC
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Allophycocyanin/Cy7
NBP2-54502APCCY7
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Biotin
NBP2-54502B
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CoraFluor 1
NBP2-54502CL1
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DyLight 350
NBP2-54502UV
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DyLight 405
NBP2-54502V
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DyLight 488
NBP2-54502G
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DyLight 550
NBP2-54502R
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DyLight 594
NBP2-54502DL594
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DyLight 650
NBP2-54502C
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DyLight 680
NBP2-54502FR
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DyLight 755
NBP2-54502IR
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FITC
NBP2-54502F
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HRP
NBP2-54502H
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Janelia Fluor 525
NBP2-54502JF525
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Janelia Fluor 549
NBP2-54502JF549
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Janelia Fluor 585
NBP2-54502JF585
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Janelia Fluor 635
NBP2-54502JF635
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Janelia Fluor 646
NBP2-54502JF646
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Janelia Fluor 669
NBP2-54502JF669
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mFluor Violet 450 SE
NBP2-54502MFV450
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mFluor Violet 500 SE
NBP2-54502MFV500
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mFluor Violet 610 SE
NBP2-54502MFV610
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PE
NBP2-54502PE
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PE/Atto594
NBP2-54502PEATT594
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PE/Cy5.5
NBP2-54502PECY55
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PE/Cy7
NBP2-54502PECY7
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PerCP
NBP2-54502PCP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # C40/1605

Concentration

0.2 mg/ml

View all CD40/TNFRSF5 Antibodies »

Product Summary for CD40/TNFRSF5 Antibody (C40/1605)

Immunogen

Recombinant full-length human CD40/TNFRSF5 protein (Uniprot: P25942)

Localization

Cell Surface and Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54502)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD40/TNFRSF5 Antibody (C40/1605)

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (C40/1605) [NBP2-53389]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (C40/1605) [NBP2-53389]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (C40/1605) [NBP2-53389] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD40/TNFRSF5 Antibody (C40/1605).
Flow Cytometry: CD40/TNFRSF5 Antibody (C40/1605) [NBP2-53389]

Flow Cytometry: CD40/TNFRSF5 Antibody (C40/1605) [NBP2-53389]

Flow Cytometry: CD40/TNFRSF5 Antibody (C40/1605) [NBP2-53389] - Flow Cytometric Analysis of U2OS cells using CD40/TNFRSF5 Antibody (C40/1605) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (C40/1605) [NBP2-53389]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (C40/1605) [NBP2-53389]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (C40/1605) [NBP2-53389] - Formalin-fixed, paraffin-embedded human Hodgkin?s Lymphoma stained with CD40/TNFRSF5 Antibody (C40/1605).
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Applications for CD40/TNFRSF5 Antibody (C40/1605)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD40/TNFRSF5

CD40 and its ligand CD154 are members of the tumor necrosis factor receptor (TNFR) and TNF families, respectively, that play key roles in signaling pathways mediating cell growth, survival and differentiation in B-lymphocytes (reviewed in Quezada et al 2004). The CD40 receptor is a 45-50 kDa glycoprotein and is expressed on the surface of B-lymphocytes, some activated T-cells, monocytes, follicular dendritic cells, basal epithelial cells, and in some epithelial and non-epithelial carcinomas. The functions of CD40 have been most extensively studied in B-cells. Ligation of B CD40 by CD154, expressed on activated T cells, stimulates B cell proliferation, differentiation, isotype switching, upregulation of surface molecules contributing to antigen presentation, development of the germinal center, and the humoral memory response. Several distinct structural motifs in the CD40 cytoplasmic domain regaulate various CD40 signaling pathways. A major CD40 signaling pathway activated from CD154 ligand binding is the canonical pathway to the transcription factor family NF-kB, a family of genes mediating immune and inflammatory responses. Although CD40 has been extensively studied as a plasma membrane-associated growth factor membrane receptor. it has also been identified in the cytoplasm and nucleus of normal and neoplastic B-lymphoid cells (Lin-Lee et al. 2006). Other growth factor receptors, including EGF, FGF, and TGF-B have also been identified in the nuclus. It is thought that plasma membrane receptor signaling may be followed by nuclear migration of signaling pathway components. The presence of CD40 in the nucleus of activated normal B lymphocytes and neoplastic B-lymphoid cells suggests that CD40 may play a more complex role in regulating essential growth and survival pathways in B-lymphocytes than previously thought.

Alternate Names

CD40, TNFRSF5

Gene Symbol

CD40

UniProt

P25942

Additional CD40/TNFRSF5 Products

Product Documents for CD40/TNFRSF5 Antibody (C40/1605)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD40/TNFRSF5 Antibody (C40/1605)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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