CD40/TNFRSF5 Antibody (C40/1605) [PE/Cy5.5]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54502PECY55

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP2-53389

Alexa Fluor 350

NBP2-54502AF350

Alexa Fluor 405

NBP2-54502AF405

Alexa Fluor 488

NBP2-54502AF488

Alexa Fluor 532

NBP2-54502AF532

Alexa Fluor 594

NBP2-54502AF594

Alexa Fluor 647

NBP2-54502AF647

Alexa Fluor 700

NBP2-54502AF700

Alexa Fluor 750

NBP2-54502AF750

Allophycocyanin

NBP2-54502APC

Allophycocyanin/Cy7

NBP2-54502APCCY7

Biotin

NBP2-54502B

CoraFluor 1

NBP2-54502CL1

DyLight 350

NBP2-54502UV

DyLight 405

NBP2-54502V

DyLight 488

NBP2-54502G

DyLight 550

NBP2-54502R

DyLight 594

NBP2-54502DL594

DyLight 650

NBP2-54502C

DyLight 680

NBP2-54502FR

DyLight 755

NBP2-54502IR

FITC

NBP2-54502F

HRP

NBP2-54502H

Janelia Fluor 525

NBP2-54502JF525

Janelia Fluor 549

NBP2-54502JF549

Janelia Fluor 585

NBP2-54502JF585

Janelia Fluor 635

NBP2-54502JF635

Janelia Fluor 646

NBP2-54502JF646

Janelia Fluor 669

NBP2-54502JF669

mFluor Violet 450 SE

NBP2-54502MFV450

mFluor Violet 500 SE

NBP2-54502MFV500

mFluor Violet 610 SE

NBP2-54502MFV610

PE

NBP2-54502PE

PE/Atto594

NBP2-54502PEATT594

PE/Cy7

NBP2-54502PECY7

PerCP

NBP2-54502PCP

Forumulation

Catalog #

Azide and BSA Free

NBP2-54502

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # C40/1605

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD40/TNFRSF5 Antibodies »

Product Summary for CD40/TNFRSF5 Antibody (C40/1605) [PE/Cy5.5]

Immunogen

Recombinant full-length human CD40/TNFRSF5 protein (Uniprot: P25942)

Localization

Cell Surface and Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Applications for CD40/TNFRSF5 Antibody (C40/1605) [PE/Cy5.5]

Application

Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CD40/TNFRSF5

CD40 and its ligand CD154 are members of the tumor necrosis factor receptor (TNFR) and TNF families, respectively, that play key roles in signaling pathways mediating cell growth, survival and differentiation in B-lymphocytes (reviewed in Quezada et al 2004). The CD40 receptor is a 45-50 kDa glycoprotein and is expressed on the surface of B-lymphocytes, some activated T-cells, monocytes, follicular dendritic cells, basal epithelial cells, and in some epithelial and non-epithelial carcinomas. The functions of CD40 have been most extensively studied in B-cells. Ligation of B CD40 by CD154, expressed on activated T cells, stimulates B cell proliferation, differentiation, isotype switching, upregulation of surface molecules contributing to antigen presentation, development of the germinal center, and the humoral memory response. Several distinct structural motifs in the CD40 cytoplasmic domain regaulate various CD40 signaling pathways. A major CD40 signaling pathway activated from CD154 ligand binding is the canonical pathway to the transcription factor family NF-kB, a family of genes mediating immune and inflammatory responses. Although CD40 has been extensively studied as a plasma membrane-associated growth factor membrane receptor. it has also been identified in the cytoplasm and nucleus of normal and neoplastic B-lymphoid cells (Lin-Lee et al. 2006). Other growth factor receptors, including EGF, FGF, and TGF-B have also been identified in the nuclus. It is thought that plasma membrane receptor signaling may be followed by nuclear migration of signaling pathway components. The presence of CD40 in the nucleus of activated normal B lymphocytes and neoplastic B-lymphoid cells suggests that CD40 may play a more complex role in regulating essential growth and survival pathways in B-lymphocytes than previously thought.

Alternate Names

CD40, TNFRSF5

Gene Symbol

CD40

Additional CD40/TNFRSF5 Products

Product Documents for CD40/TNFRSF5 Antibody (C40/1605) [PE/Cy5.5]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for CD40/TNFRSF5 Antibody (C40/1605) [PE/Cy5.5]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

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