CD44 Antibody (CD44v9/1459) - variant 9
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53204
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # CD44v9/1459
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment corresponding to the v9 domain of human CD44 (Uniprot: P16070)
Localization
Cell surface
Specificity
This antibody recognizes an epitope encoded by exon v9 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA.The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.
Marker
Marker of Tumor Metastasis
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54571)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD44 Antibody (CD44v9/1459) - variant 9
Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204]
Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204] - Formalin-fixed, paraffin-embedded human Tongue Carcinoma stained with CD44 Antibody (CD44v9/1459) - variant 9.Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204]
Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204] - Formalin-paraffin human Cervical Carcinoma stained with CD44 Monoclonal Antibody (CD44v9/1459)Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204]
Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204] - Formalin-paraffin human Prostate Carcinoma stained with CD44 Monoclonal Antibody (CD44v9/1459)Applications for CD44 Antibody (CD44v9/1459) - variant 9
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Use in WB reported in scientific literature (PMID:34490820)
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NBP2-53204 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD44
Alternate Names
CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1
Gene Symbol
CD44
UniProt
Additional CD44 Products
Product Documents for CD44 Antibody (CD44v9/1459) - variant 9
Product Specific Notices for CD44 Antibody (CD44v9/1459) - variant 9
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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