CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54571
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # CD44v9/1459
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment corresponding to the v9 domain of human CD44 (Uniprot: P16070)
Localization
Cell surface
Specificity
This antibody recognizes an epitope encoded by exon v9 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA.The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.
Marker
Marker of Tumor Metastasis
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53204).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free
Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free [NBP2-54571]
Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) [NBP2-54571] - Formalin-paraffin human Prostate Carcinoma stained with CD44 Monoclonal Antibody (CD44v9/1459)Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free [NBP2-54571]
Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) [NBP2-54571] - Formalin-paraffin human Cervical Carcinoma stained with CD44 Monoclonal Antibody (CD44v9/1459)Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free [NBP2-54571]
Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) [NBP2-54571] - Formalin-paraffin human Tongue Carcinoma stained with CD44 Monoclonal Antibody (CD44v9/1459)Applications for CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD44
Alternate Names
CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1
Gene Symbol
CD44
Additional CD44 Products
Product Documents for CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free
Product Specific Notices for CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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