CD45RB Antibody (SPM569)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32938
![Western Blot: CD45RB Antibody (SPM569) [NBP2-32938]](https://resources.bio-techne.com/images/products/CD45RB-Antibody-SPM569-Western-Blot-NBP2-32938-img0004.jpg "Western Blot: CD45RB Antibody (SPM569) [NBP2-32938]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM569
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Human peripheral blood lymphocytes maintained in T cell growth factor
Localization
Cell surface and cytoplasmic
Specificity
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Marker
Leucocyte Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34803)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD45RB Antibody (SPM569)
Western Blot: CD45RB Antibody (SPM569) [NBP2-32938]
Western Blot: CD45RB Antibody (SPM569) [NBP2-32938] - Western Blot Analysis of human spleen tissue lysates using CD45RB Antibody (SPM569).![Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) [NBP2-32938]](https://resources.bio-techne.com/images/products/CD45RB-Antibody-SPM569-Immunohistochemistry-Paraffin-NBP2-32938-img0003.jpg "Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) [NBP2-32938]")
Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) [NBP2-32938]
Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) [NBP2-32938] - Formalin-fixed, paraffin-embedded human lymphoma stained with CD45RB Monoclonal Antibody (SPM569)
Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) [NBP2-32938] -
Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) [NBP2-32938] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD45RB MAb (SPM569).Applications for CD45RB Antibody (SPM569)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD45RB
Long Name
CD45R, B isoform
Alternate Names
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbol
PTPRC
UniProt
Additional CD45RB Products
Product Documents for CD45RB Antibody (SPM569)
Product Specific Notices for CD45RB Antibody (SPM569)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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