CD45RB Antibody (SPM569) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34803

Novus Biologicals, part of Bio-Techne
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NBP2-34803-0.1mg
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM569

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all CD45RB Antibodies »

Product Specifications

Immunogen

Human peripheral blood lymphocytes maintained in T cell growth factor

Localization

Cell surface and cytoplasmic

Specificity

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

Marker

Leucocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32938).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD45RB Antibody (SPM569) - Azide and BSA Free

Western Blot: CD45RB Antibody (SPM569)Azide and BSA Free [NBP2-34803]

Western Blot: CD45RB Antibody (SPM569)Azide and BSA Free [NBP2-34803]

Western Blot: CD45RB Antibody (SPM569) - Azide and BSA Free [NBP2-34803] - Western Blot Analysis of human spleen tissue lysates using CD45RB Antibody (SPM569).
Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) - Azide and BSA Free [NBP2-34803]

Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) - Azide and BSA Free [NBP2-34803]

Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) - Azide and BSA Free [NBP2-34803] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD45RB MAb (SPM569).
Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) - Azide and BSA Free [NBP2-34803]

Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) - Azide and BSA Free [NBP2-34803]

Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) - Azide and BSA Free [NBP2-34803] - Formalin-fixed, paraffin-embedded human lymphoma stained with CD45RB Monoclonal Antibody (SPM569)

Applications for CD45RB Antibody (SPM569) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1.0ug/ml

Western Blot

0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD45RB

CD45RB is an alternatively spliced form of CD45, a cell surface glycoprotein. It is expressed on peripheral B cells, T cytotoxic cells and CD4+ T cells. It has a role in cell signalling regulation through the activation of the Src family of tyrosine kinases.

Long Name

CD45R, B isoform

Alternate Names

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbol

PTPRC

Additional CD45RB Products

Product Documents for CD45RB Antibody (SPM569) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD45RB Antibody (SPM569) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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