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CD8 beta Antibody (H35-17.2) [Alexa Fluor® 532]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08222AF532

Novus Biologicals, part of Bio-Techne
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NBP3-08222AF532

Key Product Details

Species Reactivity

Validated:

Mouse, Human (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Alexa Fluor 532

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # H35-17.2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for CD8 beta Antibody (H35-17.2) [Alexa Fluor® 532]

Immunogen

Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c (Uniprot: P10300)

Reactivity Notes

Does not react with Human.

Localization

Cell Surface and Secreted

Specificity

This monoclonal antibody reacts with the Mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Applications for CD8 beta Antibody (H35-17.2) [Alexa Fluor® 532]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD8 beta

CD8b antigen is also known as T8, Lyt2, Ly-2, and CD8 beta. It is a member of the immunoglobulin superfamily expressed on most thymocytes, some mature T cell subsets, and macrophages. CD8b forms heterodimers with the CD8 alpha chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).

Alternate Names

CD8 beta, CD8b, CD8B1, Leu2, LY3, LYT3, P37

Gene Symbol

CD8B

Additional CD8 beta Products

Product Documents for CD8 beta Antibody (H35-17.2) [Alexa Fluor® 532]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD8 beta Antibody (H35-17.2) [Alexa Fluor® 532]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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