CD8 beta Antibody (H35-17.2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08222
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse, Human (Negative)
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2b Kappa Clone # H35-17.2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for CD8 beta Antibody (H35-17.2) - Azide and BSA Free
Immunogen
Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c (Uniprot: P10300)
Reactivity Notes
Does not react with Human.
Localization
Cell Surface and Secreted
Specificity
This monoclonal antibody reacts with the Mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07331).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for CD8 beta Antibody (H35-17.2) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD8 beta
Alternate Names
CD8 beta, CD8b, CD8B1, Leu2, LY3, LYT3, P37
Gene Symbol
CD8B
Additional CD8 beta Products
Product Documents for CD8 beta Antibody (H35-17.2) - Azide and BSA Free
Product Specific Notices for CD8 beta Antibody (H35-17.2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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