CD8 beta Antibody (H35-17.2) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08222CL1
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse, Human (Negative)
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
CoraFluor 1
Antibody Source
Monoclonal Rat IgG2b Kappa Clone # H35-17.2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CD8 beta Antibody (H35-17.2) [CoraFluor™ 1]
Immunogen
Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c (Uniprot: P10300)
Reactivity Notes
Does not react with Human.
Localization
Cell Surface and Secreted
Specificity
This monoclonal antibody reacts with the Mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for CD8 beta Antibody (H35-17.2) [CoraFluor™ 1]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: CD8 beta
Alternate Names
CD8 beta, CD8b, CD8B1, Leu2, LY3, LYT3, P37
Gene Symbol
CD8B
Additional CD8 beta Products
Product Documents for CD8 beta Antibody (H35-17.2) [CoraFluor™ 1]
Product Specific Notices for CD8 beta Antibody (H35-17.2) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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