CD81 Antibody (1.3.3.22)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53318

Novus Biologicals, part of Bio-Techne
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NBP2-53318-20ug
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Single Cell Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1.3.3.22

Concentration

0.2 mg/ml

View all CD81 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human CD81 protein (Uniprot: P60033)

Localization

Cell surface

Specificity

Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54548)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD81 Antibody (1.3.3.22)

Knockout Validated: CD81 Antibody (1.3.3.22) [NBP2-53318]

Knockout Validated: CD81 Antibody (1.3.3.22) [NBP2-53318]

Knockout Validated: CD81 Antibody (1.3.3.22) [NBP2-53318] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP2-53318) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.
Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318]

Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318]

Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).
Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318]

Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318]

Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318] - Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or exosomes stained with CF568-labeled CD81 monoclonal antibody (1.3.3.22) (orange).
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Applications for CD81 Antibody (1.3.3.22)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

2-4 ug/ml

Single Cell Western

100 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 2 hours at RT.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD81

CD81/TAPA-1 is an integral membrane protein expressed on a variety of cell types, and has a high degree of sequence homology between human and mouse. (1) CD81 is expressed on thymic stromal cells where it plays an important role in the transition of gamma delta + T cells to more mature T cells with alpha beta T cell receptors. (2) Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor, which is composed of a TCR beta chain and glycoprotein pT alpha. (2) The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro. (2)

Alternate Names

CD81, TAPA-1, TSPAN28

Gene Symbol

CD81

UniProt

P60033

Additional CD81 Products

Product Documents for CD81 Antibody (1.3.3.22)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD81 Antibody (1.3.3.22)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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