CD81 Antibody (1.3.3.22)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53318
![Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318]](https://resources.bio-techne.com/images/products/CD81-Antibody-1-3-3-22-Immunohistochemistry-Paraffin-NBP2-53318-img0001.jpg "Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318]")
![Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318]](https://resources.bio-techne.com/images/products/CD81-Antibody-1-3-3-22-Flow-Cytometry-NBP2-53318-img0006.jpg "Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318]")
![Knockout Validated: CD81 Antibody (1.3.3.22) [NBP2-53318]](https://resources.bio-techne.com/images/products/CD81-Antibody-1-3-3-22-Knockout-Validated-NBP2-53318-img0005.jpg "Knockout Validated: CD81 Antibody (1.3.3.22) [NBP2-53318]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Single Cell Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1.3.3.22
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human CD81 protein (Uniprot: P60033)
Localization
Cell surface
Specificity
Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54548)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD81 Antibody (1.3.3.22)
Knockout Validated: CD81 Antibody (1.3.3.22) [NBP2-53318]
Knockout Validated: CD81 Antibody (1.3.3.22) [NBP2-53318] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP2-53318) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.![Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318]](https://resources.bio-techne.com/images/products/CD81-Antibody-1-3-3-22-Immunohistochemistry-Paraffin-NBP2-53318-img0002.jpg "Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318]")
Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318]
Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).![Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318]](https://resources.bio-techne.com/images/products/CD81-Antibody-1-3-3-22-Flow-Cytometry-NBP2-53318-img0007.jpg "Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318]")
Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318]
Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318] - Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or exosomes stained with CF568-labeled CD81 monoclonal antibody (1.3.3.22) (orange).Applications for CD81 Antibody (1.3.3.22)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
2-4 ug/ml
Single Cell Western
100 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 2 hours at RT.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 2 hours at RT.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD81
Additional CD81 Products
Product Documents for CD81 Antibody (1.3.3.22)
Product Specific Notices for CD81 Antibody (1.3.3.22)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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