CD81 Antibody (1.3.3.22) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54548
![Flow Cytometry: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548]](https://resources.bio-techne.com/images/products/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54548-img0006.jpg "Flow Cytometry: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548]")
![Knockout Validated: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548]](https://resources.bio-techne.com/images/products/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Knockout-Validated-NBP2-54548-img0005.jpg "Knockout Validated: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548]")
![Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548]](https://resources.bio-techne.com/images/products/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54548-img0001.jpg "Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548]")
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Single Cell Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1.3.3.22
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for CD81 Antibody (1.3.3.22) - Azide and BSA Free
Immunogen
Recombinant full-length human CD81 protein (Uniprot: P60033)
Localization
Cell surface
Specificity
Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53318).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD81 Antibody (1.3.3.22) - Azide and BSA Free
Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548]
Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - Formalin-fixed, paraffin-embedded human Lymph Node stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).![Flow Cytometry: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548]](https://resources.bio-techne.com/images/products/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54548-img0007.jpg "Flow Cytometry: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548]")
Flow Cytometry: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548]
Flow Cytometry: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or exosomes stained with CF568-labeled CD81 monoclonal antibody (1.3.3.22) (orange).![Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548]](https://resources.bio-techne.com/images/products/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54548-img0002.jpg "Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548]")
Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548]
Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).Applications for CD81 Antibody (1.3.3.22) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5 - 1 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1 ug/ml
Western Blot
0.5 - 1.0 ug/ml
Application Notes
Functional Studies: Order Ab without Azide.
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 2 hours at RT.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD81
Alternate Names
CD81, TAPA-1, TSPAN28
Gene Symbol
CD81
Additional CD81 Products
Product Documents for CD81 Antibody (1.3.3.22) - Azide and BSA Free
Product Specific Notices for CD81 Antibody (1.3.3.22) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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