CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44877
![Flow (Cell Surface): CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free [NBP1-44877]](https://resources.bio-techne.com/images/products/CD90-Thy1-Antibody-1aG4-C5-Azide-Free-Flow-Cell-Surface-NBP1-44877-img0002.jpg "Flow (Cell Surface): CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free [NBP1-44877]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse, Human (Negative), Rat (Negative)
Applications
CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # 1aG4/C5
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
thymocytes from C3H/Di mice (alelic form: Thy-1.2 positive)
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
The antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90); it does not cross-react with Thy-1.1 alloantigen. Thy-1 is a 18 kDa cell surface GPI linked glycoprotein (Immunoglobulin superfamily); in the mouse it is expressed on all thymocytes and peripheral T lymphocytes.
Marker
Mesenchymal Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free
Flow (Cell Surface): CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free [NBP1-44877]
Flow (Cell Surface): CD90/Thy1 Antibody (1aG4/C5) - Azide Free [NBP1-44877] - Surface staining of murine splenocytes using anti-CD90.2 (1aG4/C5) PE.![Flow (Cell Surface): CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free [NBP1-44877]](https://resources.bio-techne.com/images/products/CD90-Thy1-Antibody-1aG4-C5-Azide-Free-Flow-Cell-Surface-NBP1-44877-img0001.jpg "Flow (Cell Surface): CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free [NBP1-44877]")
Flow (Cell Surface): CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free [NBP1-44877]
Flow (Cell Surface): CD90/Thy1 Antibody (1aG4/C5) - Azide Free [NBP1-44877] - Surface staining of murine splenocytes using anti-CD90.2 (1aG4/C5) purified, DAR-PE.Applications for CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1 ug/ml
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Western Blot
1:1000
Application Notes
Functional Application: The bound of antibody 1aG4/C5 to the transfected mast cells induces the activation processes which are similar, but not identical, to the antigen activation of IgE stimulated cells. Other studies approve that the antibody 1aG4/C5 precipitates complexes with Lyn protein tyrosine kinase. This antibody is CyTOF ready.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 0.2 um filter sterilized
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD90/Thy1
Alternate Names
CD90, Thy1
Gene Symbol
THY1
Additional CD90/Thy1 Products
Product Documents for CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free
Product Specific Notices for CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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