CD90/Thy1 Antibody (1aG4/C5) [Janelia Fluor® 646]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44877JF646

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Mouse, Human (Negative), Rat (Negative)

Applications

CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Janelia Fluor 646

Antibody Source

Monoclonal Mouse IgG3 Clone # 1aG4/C5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD90/Thy1 Antibodies »

Product Specifications

Immunogen

thymocytes from C3H/Di mice (alelic form: Thy-1.2 positive)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

The antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90); it does not cross-react with Thy-1.1 alloantigen. Thy-1 is a 18 kDa cell surface GPI linked glycoprotein (Immunoglobulin superfamily); in the mouse it is expressed on all thymocytes and peripheral T lymphocytes.

Marker

Mesenchymal Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Applications for CD90/Thy1 Antibody (1aG4/C5) [Janelia Fluor® 646]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow (Cell Surface)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD90/Thy1

CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.

Alternate Names

CD90, Thy1

Gene Symbol

THY1

Additional CD90/Thy1 Products

Product Documents for CD90/Thy1 Antibody (1aG4/C5) [Janelia Fluor® 646]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD90/Thy1 Antibody (1aG4/C5) [Janelia Fluor® 646]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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