CDK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15841

Novus Biologicals, part of Bio-Techne
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NBP2-15841
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Fish

Cited:

Bovine

Predicted:

Chicken (95%), Mouse (98%), Porcine (98%), Rat (98%), Sheep (98%), Xenopus (91%), Zebrafish (92%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDK2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDK2. The exact sequence is proprietary.

Reactivity Notes

Japanese Medaka (100%), Xenopus laevis (91%). Bovine reactivity reported in scientific literature (PMID:31987327).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CDK2 Antibody

Western Blot: CDK2 Antibody [NBP2-15841]

Western Blot: CDK2 Antibody [NBP2-15841]

Western Blot: CDK2 Antibody [NBP2-15841] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human CD2K-transfected 293T cells 12 % SDS-PAGE CDK2 antibody [N1C3] dilution: 1:5000
Knockout Validated: CDK2 Antibody [NBP2-15841]

Knockout Validated: CDK2 Antibody [NBP2-15841]

Knockout Validated: CDK2 Antibody [NBP2-15841] - Wild-type (WT) and CDK2 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

Applications for CDK2 Antibody

Application
Recommended Usage

Western Blot

1:500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CDK2

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq]

Long Name

Cyclin-dependent Kinase 2

Alternate Names

cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)

Gene Symbol

CDK2

UniProt

P24941

Additional CDK2 Products

Product Documents for CDK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CDK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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