CDK2 [p Thr160] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13301

Novus Biologicals, part of Bio-Techne
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NBP3-13301
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Chicken (100%), Mouse (100%), Porcine (100%), Rat (100%), Sheep (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDK2 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr160 of human CDK2. The exact sequence is proprietary.

Modification

p Thr160

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for CDK2 [p Thr160] Antibody

Western Blot: CDK2 [p Thr160] Antibody [NBP3-13301]

Western Blot: CDK2 [p Thr160] Antibody [NBP3-13301]

Western Blot: CDK2 [p Thr160] Antibody [NBP3-13301] - Wild-type (WT) and CDK2 (phospho Thr160) knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Thr160) antibody (NBP3-13301) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-13301]

Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-13301]

Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-13301] - CDK2 (phospho Thr160) antibody detects CDK2 (phospho Thr160) protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human colon. CDK2 (phospho Thr160) stained by CDK2 (phospho Thr160) antibody (NBP3-13301) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: CDK2 [p Thr160] Antibody [NBP3-13301]

Western Blot: CDK2 [p Thr160] Antibody [NBP3-13301]

Western Blot: CDK2 [p Thr160] Antibody [NBP3-13301] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Thr160) antibody (NBP3-13301) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
View 1 more images

Applications for CDK2 [p Thr160] Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDK2

The protein encoded by the Cdk2 gene is a member of the Ser/Thr protein kinase family. This protein kinase is highlysimilar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of thecyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycleG1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex includingcyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its proteinphosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have beenreported. (provided by RefSeq)

Long Name

Cyclin-dependent Kinase 2

Alternate Names

cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)

Gene Symbol

CDK2

Additional CDK2 Products

Product Documents for CDK2 [p Thr160] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CDK2 [p Thr160] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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