CEACAM1/CD66a Antibody (PSH0-78)

Western Blot: CEACAM1/CD66a Antibody (PSH0-78) [NBP3-32177]

Western Blot: CEACAM1/CD66a Antibody (PSH0-78) [NBP3-32177] - Western blot analysis of CEACAM1/CD66a on different lysates with Rabbit anti-CEACAM1/CD66a antibody (NBP3-32177) at 1/1,000 dilution and Mouse anti-6X His-tag antibody (HA600030) at 1/1,000 dilution.

Lane 1: His-tagged human CEACAM-1 recombinant protein aa (aa35-428) 20ng
Lane 2: His-tagged human CEACAM-5 recombinant protein aa (aa35-422) 20ng
Lane 3: His-tagged human CEACAM-6 recombinant protein aa (aa35-326) 20ng
Lane 4: His-tagged human CEACAM-8 recombinant protein aa (aa35-335) 20ng

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32177) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: CEACAM1/CD66a Antibody (PSH0-78) [NBP3-32177]

Immunohistochemistry: CEACAM1/CD66a Antibody (PSH0-78) [NBP3-32177] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CEACAM1/CD66a antibody (NBP3-32177) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32177) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.