Chk2 Antibody (73C175.1.1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56546
![Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546]](https://resources.bio-techne.com/images/products/Chk2-Antibody-73C175-1-1-Western-Blot-NB100-56546-img0003.jpg)
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 73C175.1.1
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A portion of amino acids 100-150 of human Chk2 protein was used as immunogen for this antibody.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Chk2 Antibody (73C175.1.1) - BSA Free
Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546]
Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (73C175.1.1) [NB100-56546]
Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) [NB100-56546] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) [NB100-56546]
Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) [NB100-56546] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.Applications for Chk2 Antibody (73C175.1.1) - BSA Free
Application
Recommended Usage
Flow Cytometry
1-5 ug/ml
Immunocytochemistry/ Immunofluorescence
2-5 ug/ml
Western Blot
2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS containing 0.2% gelatin
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Chk2
Long Name
Checkpoint Kinase 2
Alternate Names
CDS1, CHEK2, HuCds1, LFS2, PP1425, Rad53
Entrez Gene IDs
11200 (Human)
Gene Symbol
CHEK2
UniProt
Additional Chk2 Products
Product Documents for Chk2 Antibody (73C175.1.1) - BSA Free
Product Specific Notices for Chk2 Antibody (73C175.1.1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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