Chk2 Antibody (8F12)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-500
Key Product Details
Species Reactivity
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 8F12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant CHK2 protein
Localization
Nuclear
Specificity
Specific for human Chk2 protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Chk2 Antibody (8F12)
![Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (8F12) [NB100-500]](https://resources.bio-techne.com/images/products/Chk2-Antibody-8F12-Immunocytochemistry-Immunofluorescence-NB100-500-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (8F12) [NB100-500]")
Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (8F12) [NB100-500]
Immunocytochemistry/Immunofluorescence: Chk2 Antibody (8F12) [NB100-500] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 1:500 dilution.
Western Blot: Chk2 Antibody (8F12) [NB100-500] -
Western Blot: Chk2 Antibody (8F12) [NB100-500] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk2 antibody [8F12] (NB100-500) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Applications for Chk2 Antibody (8F12)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500
Western Blot
1:500-1:3000
Application Notes
WB: Detects Chk2 protein in approx. 20 - 40 ug total cell extract at 1:1000 dilution
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.2)
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Chk2
Long Name
Checkpoint Kinase 2
Alternate Names
CDS1, CHEK2, HuCds1, LFS2, PP1425, Rad53
Entrez Gene IDs
11200 (Human)
Gene Symbol
CHEK2
Additional Chk2 Products
Product Documents for Chk2 Antibody (8F12)
Product Specific Notices for Chk2 Antibody (8F12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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