Chk2 [p Thr68] Antibody (JE43-12)

Western Blot: Chk2 [p Thr68] Antibody (JE43-12) [NBP3-32756]

Western Blot: Chk2 [p Thr68] Antibody (JE43-12) [NBP3-32756] - Western blot analysis of Chk2 on different lysates with Rabbit anti-Chk2 antibody (NBP3-32756) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 20uM Etoposide for 2 hours cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Jurkat treated with 25uM Etoposide for 5 hours cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: NIH/3T3 treated with 25uM Etoposide for 5 hours cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 61 kDa
Observed band size: 61 kDa

Exposure time: 5 minutes;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32756) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.